Lenti-mEGFP-hsG3BP1 delet NTF2L

LOCUS       Lenti-mEGFP-hsG3BP1 delet NTF2L              9919 bp DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..9919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2202..2209
                     /label=enzyme cut site
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    complement(3461..3499)
                     /label=Primer R
     misc_feature    3479..3517
                     /label=Primer F
     CDS             3497..4486
                     /codon_start=1
                     
                     /translation="FGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSND
                     MEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQT
                     VQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD
                     QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDF
                     FQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAA
                     REGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ"
     misc_feature    4487..4492
                     /label=enzyme cut site
     promoter        4497..4996
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4532..4555)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4929..4948
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5023..5817
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5077..5096)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5687..5706
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5841..6429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5894..5914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6312..6323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6637..7270
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7399..7415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7399..7415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7412..7434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7423..7439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7447..7477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7492..7513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7630..7647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7801..8386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7881..7900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8557..9417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9180..9199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9418..9522)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9570..9704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9607..9626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9661..9680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctctttg gtgggtttgt cactgagcct caggaggagt ctgaagaaga
     3541 agtagaggaa cctgaagaaa gacagcaaac acctgaggtg gtacctgatg attctggaac
     3601 tttctatgat caggcagttg tcagtaatga catggaagaa catttagagg agcctgttgc
     3661 tgaaccagag cctgatcctg aaccagaacc agaacaagaa cctgtatctg aaatccaaga
     3721 ggaaaagcct gagccagtat tagaagaaac tgcccctgag gatgctcaga agagttcttc
     3781 tccagcacct gcagacatag ctcagacagt acaggaagac ttgaggacat tttcttgggc
     3841 atctgtgacc agtaagaatc ttccacccag tggagctgtt ccagttactg ggataccacc
     3901 tcatgttgtt aaagtaccag cttcacagcc ccgtccagag tctaagcctg aatctcagat
     3961 tccaccacaa agacctcagc gggatcaaag agtgcgagaa caacgaataa atattcctcc
     4021 ccaaagggga cccagaccaa tccgtgaggc tggtgagcaa ggtgacattg aaccccgaag
     4081 aatggtgaga caccctgaca gtcaccaact cttcattggc aacctgcctc atgaagtgga
     4141 caaatcagag cttaaagatt tctttcaaag ttatggaaac gtggtggagt tgcgcattaa
     4201 cagtggtggg aaattaccca attttggttt tgttgtgttt gatgattctg agcctgttca
     4261 gaaagtcctt agcaacaggc ccatcatgtt cagaggtgag gtccgtctga atgtcgaaga
     4321 gaagaagact cgagctgcca gggaaggcga ccgacgagat aatcgccttc ggggacctgg
     4381 aggccctcga ggtgggctgg gtggtggaat gagaggccct ccccgtggag gcatggtgca
     4441 gaaaccagga tttggagtgg gaagggggct tgcgccacgg cagtgagaat tctaccgggt
     4501 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     4561 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     4621 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     4681 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     4741 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     4801 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     4861 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     4921 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     4981 atctccgggc ctttcgacct gcagcccaag cttcctagga ccatgattga acaagatgga
     5041 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5101 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5161 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     5221 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5281 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5341 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5401 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5461 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5521 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     5581 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5641 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5701 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     5761 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaccc
     5821 gggcggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5881 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5941 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6001 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6061 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6121 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6181 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6241 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6301 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6361 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6421 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     6481 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6541 ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6601 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     6661 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     6721 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     6781 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     6841 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     6901 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     6961 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7021 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     7081 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7261 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7321 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta
     7381 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     8341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8521 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9541 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
     9601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     9721 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag
     9781 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9841 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
     9901 gtactacaaa cttagtagt
//